Table 4.

The content of phosphorus in the yeast strains

Strains	PO4 of elutiona (mg P/l·OD660)	Poly-P of elutiona (mg P/l·OD660)	T-P of elutiona (mg P/l·OD660)	T-P of cellsb (mg P/l·OD660)
H. fabianii J640 (wild strain)	0.35	0.59	1.12	1.89
H. fabianii J640 PFW1	0.64	2.88	3.50	6.59
H. fabianii J640 PFW2	0.54	2.76	3.32	5.29
H. fabianii J640 PFW3	0.49	3.16	3.67	6.46
H. fabianii J640 PFW4	0.71	2.33	2.98	4.71
H. anomala J224-1 (wild strain)	0.55	1.30	2.09	3.86
H. anomala J224-1 PAW1	0.38	4.03	4.60	8.34

Each strain was incubated in 5 ml of high-P YPD medium with shaking for 24 h.

^aElution of yeast cells following heating at 70°C for 1 h. PO₄-P and T-P were assayed by standard methods; poly-P was hydrolyzed to PO₄-P by heating at 100°C for 7 min with 1 N HCl, then assayed by standard methods.

^bTotal phosphorus content of the yeast cells was assayed by standard methods.