FIGURE 3.

Effect of miRNA inhibition on EMCV IRES-driven translation. (A) Depiction of the relative rates used in modeling cap-dependent and -independent (EMCV IRES) driven translation. The size of the arrow corresponds to the magnitude of the rate indicated. Panels B–I display the results of modeling potential sites of miRNA action on IRES and cap-dependent translation depicting protein production. The region of rates inhibited by miRNA is from 0% to 100% repression of the respective values, where 100% repression results in no protein production. The circles on m7G cap lines indicate the observed in vitro miRNA-mediated inhibition in Mathonnet et al. (2007). The circles on the IRES lines indicate the amount of translation if repressed equivalently to the m7G mRNA. (B–C) Protein production from increasing repression of cap-dependent k1 shown as total and relative amounts, respectively. (D–E) Protein production from increasing repression of cap-independent k1 shown as total and relative amounts, respectively. (F–G) Protein production from increasing repression of k2 shown as total and relative amounts, respectively. (H–I) Protein production from increasing repression of k3 shown as total and relative amounts, respectively.