Table 2.

Amino acid differences between human and murine thrombin.

	1e	13	14a	14d	14h	20	24	26	27	59	79
Human	S	E	K	R	E	S	I	M	S	L	I
Murine	L	K	T	K	D	W	K	I	A	I	V
	90	106	126	127	129	129a	131	145	149a	149c	149d
Human	I	M	R	E	A	A	Q	K	A	V	G
Murine	V	L	K	Q	V	T	R	R	T	I	N
	149e	150	170	184a	186	186a	186b	186c	222	236
Human	K	G	D	Y	P	D	E	G	D	K
Murine	E	I	A	F	V	N	D	T	K	R

Replacements in italic (9 total) indicate non-conservative charge substitutions. The thrombin chimera was constructed by replacing residues 184a, 186, 186a, 186b, 186c and 222 in human thrombin with those of the murine enzyme.