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. 2008 Aug 13;3(8):e2938. doi: 10.1371/journal.pone.0002938

Table 1. Distribution of the 11973G/A mitochondrial heteroplasmy in 20 isopod crustacean species.

SUBORDER, Family Species Origin Na 11973b Assayc PCR primersd
FLABELLIFERA
Sphaeromatidae Dynamene bidentata France 2 G F, D VF2/VR2
ONISCIDEA
Ligiidae Ligia oceanica France 3 G F, R, D VF1/1737ND
Philosciidae Chaetophiloscia elongata France 2 G F, R, D VF1/1737ND
Philoscia muscorum France 2 G F, R, D VF1/1737ND
Agnaridae Hemilepistus reaumuri Tunisia 3 G/A F, R, D VF2/VR2
Armadillidae Armadillo officinalis Turkey 2 G/A F, R VF2/VR2
Cubaris murina Guadeloupe 2 G/A F, R, D VF2/VR2
Armadillidiidae Armadillidium assimile France 3 G/A F, R, D 2138ND/1737ND
Armadillidium depressum France 3 G/A F, R, D 2138ND/1737ND
Armadillidium nasatum France 2 G/A F, R, D VF1/1737ND
Armadillidium vulgare Brazil, France, Greece, Tunisia 26 G/A F, R, D 2138ND/1737ND
Balloniscidae Balloniscus sellowii Brazil 1 G/A F, R, D VF2/VR2
Cylisticidae Cylisticus convexus France 3 G/A F, R, D VF2/VR2
Platyarthridae Platyarthrus hoffmannseggii France 1 G/A F, R VF2/VR2
Platyarthrus caudatus Italy 1 G/A F, R VF2/VR2
Porcellionidae Porcellio gallicus France 3 G/A F, R, D VF2/VR2
Porcellio spinicornis France 2 G/A F, R, D VF2/VR2
Trachelipodidae Trachelipus rathkii France 2 G/A F, R, D VF1/1737ND
Trichoniscidae Trichoniscus pusillus pusillus France 1 G/A F, R VF2/VR2
Tylidae Helleria breviconis France 2 G/A R, D VF2/VR2
a

Number of individuals tested.

b

Indicates whether all individuals within species were homoplasmic for guanine (G) or heteroplasmic for guanine and adenine (G/A) at mitochondrial genome position 11973.

c

Assay used to ascertain 11973 heteroplasmic status: direct sequencing with forward (F) or reverse (R) primer, and HpyCH4V digestion (D).

d

Primer pairs used to amplify the 11973 mitochondrial genome region by PCR: VF1 (5′-CCCGTTTGAGTGTGGGTTTGA), VF2 (5′- TGGTTTTTGATGTTGAGATT), 2138ND (5′-TCCTAGGGATTGGCCATTTA), VR2 (5′- CGCTTACGTTACGATAAACT) and 1737ND (5′- TATTTGGGTGCGAGGAACTC).