Table 5. Immunogenicity of the pan-DENV sequences in HLA-DR transgenic mice.
| DENV protein | Pan-DENV sequenceb | Ag-specific CD4 T-cell responsesa | |||
| Peptide sequences (DENV-3)c | IFN-γ-SFC/106splenocytes±SDd | ||||
| DR2 | DR3 | DR4 | |||
| E | 252VLGSQEGAMH261 | PEVVVLGSQEGAMHT | - | - | 88±34 |
| NS1 | 193AVHADMGYWIES204 | AVHADMGYWIESQKN | - | 17±1 | - |
| 229HTLWSNGVLES239 | WPKSHTLWSNGVLES | - | 129±3* | - | |
| HTLWSNGVLESDMII | - | 131±103 | 37±3 | ||
| 266GPWHLGKLE274 | HTQTAGPWHLGKLE | - | 333±6 | - | |
| 294RGPSLRTTT302 | TRGPSLRTTTVSGKL | - | - | 11±4 | |
| NS3 | 189LTIMDLHPG197 | KKRNLTIMDLHPGSG | - | - | 50±16 |
| 296AARGYISTRV305 | ASIAARGYISTRVGM | 40±14 | - | - | |
| ARGYISTRVGMGEAA | 9±4 | - | - | ||
| 313IFMTATPPG321 | EAAAIFMTATPPGTA | - | - | 474±116 | |
| IFMTATPPGTADAFP | - | - | 323±287 | ||
| 357GKTVWFVPSIK367 | TDFAGKTVWFVPSIK | 48±15 | - | - | |
| GKTVWFVPSIKAGND | 396±14 | - | - | ||
| 383VIQLSRKTFD392 | KKVIQLSRKTFDTEY | - | 21±3 | - | |
| 406VVTTDISEMGANF418 | FVVTTDISEMGANFK | - | - | 408±104 | |
| TDISEMGANFKADRV | - | 152±33 | - | ||
| NS5 | 302TWAYHGSYE310 | DENPYKTWAYHGSYEVK | 126±10* | - | 14±5 |
| TWAYHGSYEVKATGSA | 161±20* | - | 63±17 | ||
| 342AMTDTTPFGQQRVFKEKVDTRT363 | MVTQMAMTDTTPFGQQR | - | - | 28±0* | |
| 450CVYNMMGKREKKLGEFG466 | GSCVYNMMGKREKKLGE | - | - | 13±2 | |
| 505SGVEGEGLH513 | NSYSGVEGEGLHKLGYI | - | - | 184±15 | |
| 531YADDTAGWDTRIT543 | KIPGGAMYADDTAGWDT | - | - | 46±3 | |
| 568IFKLTYQNKVV578 | ANAIFKLTYQNKVVKVQ | 577±384 | - | 24±9* | |
| 597DQRGSGQVGTYGLNTFTNME616 | VMDIISRKDQRGSGQVG | - | 88±1 | - | |
| 658RMAISGDDCVVKP670 | VERLKRMAISGDDCVVK | - | 159±24 | 16±6 | |
| MAISGDDCVVKPIDDRF | - | 249±39 | - | ||
| 707VPFCSHHFH715 | DWQQVPFCSHHFHELIM | 32±8* | 34±11 | - | |
| 765LMYFHRRDLRLA776 | MYFHRRDLRLASNAI | 75±16* | - | 33±9 | |
| 790PTSRTTWSIHA800 | VHWVPTSRTTWSIHAHH | - | - | 83±1 | |
| SRTTWSIHAHHQWMTTE | - | - | 122±46 | ||
a
Assessed by IFN-γ ELISpot assay in HLA-DR2 (DRB1*1501), HLA-DR3 (DRB1*0301) and HLA-DR4 (DRB1*0401) Tg mice immunized with DENV-3 peptides (see Methods)
b
Amino acid positions numbered according to the sequence alignments of the 4 DENV types
c
Sequences present in the pan-DENV sequences are underlined
d
SFC, spot-forming cells; SD, standard deviation. Representative results from at least two immunized Tg mice are shown, except when indicated by an asterisk