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. 1998 Nov 10;95(23):13835–13840. doi: 10.1073/pnas.95.23.13835

Figure 1.

Figure 1

Expression and activity of the OG2-Tk transgene. (A) Schematic representation of the transgene (Upper) and Northern blot analysis of its expression (Lower). (B) In vitro osteoblast ablation by GCV in calvarial cell culture of tg mice. Northern blot analysis shows osteocalcin (OC) expression in cells cultured without GCV for 4 days (−) and in the presence of 25 μM GCV for 4 days (4d) and 8 days (8d). Equivalent amounts of intact RNA were run in each lane as indicated by hybridization to an 18S RNA probe. (C) The graph shows in vivo osteoblast ablation after GCV treatment: measurement of circulating osteocalcin (OC) in wt animals (dotted line, n = 6) or tg animals (solid line, n = 6) before (6 weeks old), at the end of (10 weeks old), or 4 weeks after the GCV treatment period (14 weeks old).