Table 2.
Primers used in this study
| Primer Designation | Primer sequence 5' – 3' | Ta (°C) | Product size (bp) |
| VPaI-2 | |||
| VP0634F | GGGGGAAATAAATGTCTGAAGG | 52.1 | 1363 |
| VP0634R | AACACGCCAAGACTCTC | ||
| VP0637F | GGAATAACTCAGAGCTTCG | 52.8 | 1848 |
| VP0640R | TAGGCAGTCGTAATTCG | ||
| VP0643F | ATACGCCTGATTGCTTC | 52.0 | 1558 |
| VP0643R | TGGTACTATCAACGCCG | ||
| VP0644F | CGTGCTTTTTCTCTTGC | 51.1 | 970 |
| VP0644R | CCATATTGCTAGTTAGCTCG | ||
| VPaI-3 | |||
| VP1069F | TAGGGTCGGTGGTGTACTTG | 52.8 | 2041 |
| VP1069R | GACTCCACTATTGGTTTA GC | ||
| VP1072F | AGAGTCAGAGGAAAGGGAGG | 50.3 | 2275 |
| VP1073R | GTAAATGTTGTGGGTGC | ||
| VP1079F | CTGTCTTCATGCCTTTG | 51.0 | 1691 |
| VP1079R | CGCCATTGCTAAACGTC | ||
| VP1083F | CTTACTTATTGGAGGCTGG | 52.1 | 2127 |
| VP1083R | GGTGGGTATAAAGGTAACG | ||
| VP1095F | TCTGGTTCGGTATTTGG | 52.7 | 1166 |
| VP1096R | CGCAGCATTTCTTGAAG | ||
| VPaI-7 | |||
| VPA1308F | TTAGAACGCATGAGCACCG | 53.1 | 1844 |
| VPA1309R | CCACCAAAGTGTTTGTGAG | ||
| VPA1312F | CTACTATCATCACGACGTG | 49.4 | 1487 |
| VPA1314R | CGTGCTTATAGCCAGAC | ||
| VPA1317F | GACAGACAGAGATACGCTG | 50.9 | 1366 |
| VPA1320R | TTCAGAGGTGTCGCACTTCG | ||
| VPA1321F | CGTGGTGGTTAGTGAATC | 49.1 | 886 |
| VPA1321R | AGAGTTGGTTTCGCAGG | ||
| VPA1321F | GACCACTATATTGTTCTCCG | 49.4 | 1480 |
| VPA1323R | CTCAGGGATAAATAGGGATG | ||
| VPA1331F | CCAATAATCACCCTCCG | 49.9 | 1857 |
| VPA1334R | CTCAGGGATAAATAGGGATG | ||
| VPA1340F | GTCCTTGATTACACCATTGG | 51.8 | 1618 |
| VPA1343R | GCACGTAACATCTAAGTTCGTG | ||
| VPA1350F | TGCATCGTCATTTCTCC | 50.4 | 2630 |
| VPA1354R | CGTAGATTTCATGGCAG | ||
| VPA1363F | TTTCACTAATGCTGCGG | 51.1 | 2308 |
| VPA1365R | GGTCAATATGGCACTATGC | ||
| VPA1380F | TTAGGGGTGTTATGCCG | 48.5 | 816 |
| VPA1380R | TTACTGTCTCTGTTGCAGG | ||
| VPA1390F | CCACAACACAAACTGTCC | 50.9 | 2606 |
| VPA1393R | AATCCAAGGGGAGTGAC | ||
| VPA1394F | AACGCCGAATTAACCGC | 53.2 | 2356 |
| VPA1395R | TCACCCCAATGTACCGTCTG | ||
| VPA1397F | GCGGAGCTGTAATGAAATG | 52.9 | 793 |
| VPA1398R | CAACCAACGTATTGTAGCAG | ||
| VPA1400F | ATAGGTCTGTGTAACCCG | 52.3 | 1964 |
| VPA1401R | GGTAAAGCTGCGATGAC | ||
| VP0085F | TGCTCGCTGCTATCTAC | 53.0 | 1160 |
| VP0085R | CGTTAAATACGCCAGTTGC | ||
| VP0220F | CCCTCAAGTGATTGATCC | 53.0 | 1878 |
| VP0220R | AAGATAGCCCCTTGTGG | ||
| VP1399F | CATCTCTTGCTCTTGGAG | 51.0 | 1312 |
| VP1400R | TGAGGTCTACAATGAGTCAG | ||
| VP1415F | CGCAATTAAAGGCAGTACG | 51.0 | 2274 |
| VP1416R | GACTGAATAAGAGTGCTCG | ||
| VP1556F | TCCCGATTGTAAGTTGC | 53.0 | 1957 |
| VP1558R | AGCTAATGCGAATGAGC | ||
| VPA0446F | ACGTTCTTTTGGGATGG | 53.0 | 2376 |
| VPA0447R | ACCGAAGCCTTAACACG | ||
| VPA0450F | AATGCGAAAGAAGGCGATAC | 60.0 | 1337 |
| VPA0450R | TGCGCTTGTAGATGAGTTGG | ||
| VPA0891F | GTCGCTCTTTATGTTGC | 50.5 | 615 |
| VPA0891R | GAAGCCTGTATCAACTGTC | ||
| VPA0894F | TAATGGTCGATGCACTG | 54.0 | 1215 |
| VPA0894R | GGATGAGCAAGTCAGTAGC | ||
| VPA0952F | CGAGTGATCCAGTTTTACAC | 52.0 | 2720 |
| VPA0953R | AACTACCAGCTAGAAGTGG | ||
| VPA0992F | CCGACATAAAGGGATACTC | 52.0 | 1926 |
| VPA0992R | GAAGAAGCACTTGCTCTC | ||
| VPA1443F | CAATCAGCAGCCAGTCGTTA | 60.0 | 1233 |
| VPA1443R | CCGGATGTCAAACGGTACTT | ||
| VPA1503F | GTTCGACAATGGCATGTGAG | 60.0 | 801 |
| VPA1503R | CGCCAGTATCGACATCACTC | ||
| VPA1655F | CCGTTTTGCTGATGCTACTG | 60.0 | 816 |
| VPA1655R | ATGACCACAGTTCCGGAGAG | ||
| VPGyrBF | GTA CTG AAG GGT CTG GAT GC | 54.6 | 742 |
| VPGyrBR | ACT GCA TTG CCA CTT CTA CC | ||
| VPmdhF | TGAAAGTAGCCGTTATTGG | 54.0 | 901 |
| VPmdhR | CCATTTAGCGTTTCTAGCATTC | ||
| VPGroEL1F | TTTCGGTGCTCCAACCATC | 55.4 | 737 |
| VPGroEL1R | GCATTGCTTTACGACGGTC | ||