Table 4.

Frequency of genes following amino acid rules specific for the PRD or ANTP classes.

Position	27		29		30		30		50
Rules	Not Ne		Not A		Not (LP, NP)		Not (LH, VH)		(P,Ne)
Dataset	Phy	Unc	Phy	Unc	Phy	Unc	Phy	Unc	Phy	Unc
PRD	0.060 (200)	0.000 (14)	0.305 (200)	0.714 (14)	0.020 (200)	0.071 (14)	0.020 (200)	0.071 (14)	0.315 (197)	0.786 (14)
(HOXL and NKL)	0.998 (406)	1.000 (230)	1.000 (406)	1.000 (230)	0.998 (406)	1.000 (230)	0.998 (406)	1.000 (230)	0.000 (404)	0.000 (230)

In brackets is indicated the total number of sequences analysed.

Phy – set of sequences classified using a phylogenetic approach

Unc – set of sequences not classified using a phylogenetic approach. These sequences, nevertheless, show amino acid patterns typical of PRD, HOXL and NKL genes. Ne – negatively charged amino acids; P – positively charged amino acids; A -aromatic amino acids; LP – Less-polar amino acids; NP – Non-polar amino acids; LH – less hydrophobic amino acids; VH – very hydrophobic amino acids; S- small amino acids; T – tinny amino acids; Al – Alyphatic amino acids.