Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
A master mixture for 96-strain transformation.
| Ingredient | Volume |
|---|---|
| 50% polyethylene glycol (PEG-3350, Sigma) | 6,240 μl |
| 1 M LiOAc | 822 μl |
| Heat-denatured herring sperm DNA (10 mg/ml) | 500 μl |
| Query construct (PCR product; 100 ng/μl) | 100 μl |
| DMSO | 936 μl |
| Total | 8,598 μl |
