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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: Clin Cancer Res. 2008 Jun 15;14(12):3966–3974. doi: 10.1158/1078-0432.CCR-08-0356

Figure 2. TGF-β Receptor Blockade Increases the Number of Transferred T-cells in spleen and lymph node.

Figure 2

Twenty million activated GFP-expressing OT-1 splenocytes were injected into tumor-bearing animals on control diets or SM16 diets. Three days after transfer, spleen and lymph nodes were isolated and subjected to FACS analysis to identify transferred CD8+/GFP+ T-cells.

Panel A shows results from Spleen and Panel B from lymph nodes. The upper panels show representative FACS tracings (CD8 on the X-axis and GFP on the Y axis) from control animals (no adoptive transfer), animals on control chow who received adoptive transfer of T cells, and animals on SM16 chow who received adoptive transfer of T cells. The transferred cells (CD8+/GFP+) are visualized in the right upper quadrant of each tracing. The lower graph shows the average values of the CD8+/GFP+ populations in each group for three independent experiments. *= p<0.05 from control and SM16 + AT; ** = p<0.05 from control and Control chow plus adoptive transfer.