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. 2008 Jul 23;4(4):202–207. doi: 10.7150/ijbs.4.202

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 2 — Identification of targeted ES clones by Southern blot analysis. A & B. Southern blot analyses of ES clones using the 5' and the 3' probes. DNA was digested with Sca I and Sal I in panel A and with Bam HI and Kpn I in panel B. The genotypes of WT (+/+) and targeted (f-neo) ES clones are indicated. C. Southern blot analysis of ES clones using the neo probe. The correctly targeted clones (1A6, 1G12, 2E8 & 3E11) contain only one neo integration site within the 7.5-kb band for the targeted SRC-3 allele. DNA was digested as that in panel B.