Figure 6. Quantitative RT PCR analysis on total RNA from cells transiently transfected with wild type E7 or codon-modified E7 expression constructs demonstrated that E7/22 message was present at about four times the numbers of copies of the other genes.

RNA was isolated from transiently transfected cells and subjected to QRT- PCR. Standard curves were run with each reaction and quantitation of message was done using these curves. E7/22 message was present at levels four fold higher than the other genes.