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. 2008 Aug 13;3(8):e2961. doi: 10.1371/journal.pone.0002961

Figure 4. HIV-induced IDO suppresses both CD4 and CD8 T cell proliferation in a 2-step experiment.

Figure 4

(A) CD4 and CD8 T cell proliferation is shown as the increase in the number of viable cells measured using a bioreduction assay. Relative cell number was calculated for each sample as ratio between stimulated (with anti-CD3 and anti-CD28) and unstimulated cells cultured in the three different conditioned media (CM). (B) CD4 and CD8 T cell apoptosis is shown as frequency of annexin V+ cells measured by flow cytometry. Apoptosis of cells cultured in the three different conditioned media (CM) is shown for both unstimulated and stimulated (with anti-CD3 and anti-CD28) cells. In all cases mean values±standard error calculated on 8 independent experiments are shown.