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(A) NF-κB induction was measured in HEK-4-Blue™ cells treated overnight with 1:2 serially diluted doses of TLR ligands of interest. Secreted alkaline phosphatase was measured colorimetrically. (B) NF-κB induction in HEK-2-Blue™ cells treated overnight with 1:2 serially diluted doses of the various ligands was similarly ascertained via a colorimetric assay.
