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. Author manuscript; available in PMC: 2009 Jun 30.
Published in final edited form as: Immunol Lett. 2008 Apr 18;118(2):132–141. doi: 10.1016/j.imlet.2008.03.009

Fig. 3.

Fig. 3

(A) NF-κB induction in 23 ScCR cells stably transfected with the pNifty plasmid expressing the NF-κB-sAP reporter gene. (B) Phosphorylation of p38 MAPK in murine macrophage 23 ScCR cells, analyzed by flow cytometry. (C) NO production was tested in 23 ScCR cells primed with IFN-γ (1000 Units/ml). (D) MIP-1α release in 23 ScCR cells upon treatment with the ligands for 4 hours, measured by CBA. (E) RANTES/CCL5 chemokine release in human blood was measured following 8 hour challenge with the ligands of interest diluted serially 1:2. The serum was analyzed for amount of chemokine released via cytometric bead array. Robust, biphasic responses are observed in the LPS- and Pam2CSK4-treated samples. Representative results from three independent experiments performed on blood samples from different donors are shown in D and E.