Figure 1.
B29 minimal promoter activity is modulated by 5′ flanking sequences. Transient transfections to detect transcriptional expression of sequential B29 promoter deletion constructs were carried out in the M12 B cell line. Deletion constructs are identified by nucleotide numbers with respect to the major start site of transcription (+1). The activity of each deletion construct is expressed as a percentage of the CAT activity obtained with the B29 minimal (−164) promoter construct (100%).
