Figure 6.

Effect of a hole trap on migration of oxidative damage in PNA/DNA. Samples containing 5.0 μM each of PNA(AQ1) and DNA-1T or DNA-3 (8-OxoG) were prepared and treated as described in Fig. 4. Conditions of irradiation and piperidine treatment are indicated above individual lanes. Lanes: 1–4, DNA-1T; 5–8, DNA-3. Note the lone cleavage band observed in lane 6; this band corresponds to the 8-OxoG site, which is cleaved slowly by piperidine treatment (23). The three GG sites are indicated at the left of the figure, with site A at the top and site C at the bottom.