Fig. 5.
Alix multimerization is required for Alix-dependent HIV-1 release. Cells (293T) were cotransfected with plasmids encoding the indicated HA-tagged AlixR constructs and an HIV-1 pNL/HXB p6(P7L, P10L) plasmid. Resolved cell lysates and released virions were examined by Western blot analysis with α-Gag antisera. Quantification of virion release (VR) via infrared imaging is given. (n = 3). Lysates were also examined by Western blot analysis with α-HA antisera. β-Galactosidase assay was performed on HeLa-TZM-bl cells infected with 293T supernatants (n = 3 ±SD).