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. 2008 Jul 22;105(30):10565–10570. doi: 10.1073/pnas.0801765105

Fig. 2.

Fig. 2.

The in vitro interaction of M-PMV wt and R55F MA with Tctex-1. The wt or R55F MA C-terminally extended with GST (MA-GST) were immobilized on GST·Bind resin (Novagen) and allowed to interact with lysates of E. coli BL21(DE3) cells expressing human Tctex-1. Proteins were resolved by SDS Tris-Tricine acrylamide gel electrophoresis. (A) Coomassie blue-stained gel. (B) Western blot; MA detected by rabbit anti-M-PMV MA antibody. (C) Western blot; Tctex-1, detected by rabbit anti-Tctex antibody. Lane 1, total lysate of E. coli BL21(DE3) after 4 h expression of Tctex-1; lane 2, binding of Tctex-1 to wt MA-GST immobilized on GST·Bind resin; lane 3, binding of Tctex-1 to MA.R55F-GST immobilized on GST·Bind resin; lane 4, Tctex-1 applied on GST·Bind resin (control of possible nonspecific Tctex-1 binding).