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. 2008 Jul 22;105(30):10565–10570. doi: 10.1073/pnas.0801765105

Fig. 3.

Fig. 3.

The interaction of M-PMV wt and R55F Gag with human Tctex-1 in the COS-1 cells. The interaction of Gag with Tctex-1 was determined by coimmunoprecipitation from the lysate of COS-1 cells cotransfected with vectors carrying genes encoding human Tctex-1 tagged with a c-myc epitope (c-mycTctexpCMV) and either wt Gag or R55F Gag as shown on top of the figure. Gag and Tctex-1 proteins were resolved by SDS/PAGE or SDS Tris-Tricine acrylamide gel electrophoresis, respectively. Rabbit anti-M-PMV CA and monoclonal anti-c-myc antibodies were used for Western blot detection of intracellular expression of Gag and Tctex-1 proteins, respectively. The complexes were immunoprecipitated with rabbit anti-CA antibody. Monoclonal anti-c-myc antibody was used for Western blot identification of immunoprecipitated Tctex-1. (A and B) Analysis of intracellular proteins. (C) Analysis of immunoprecipitated material.