Fig. 3.

Structural dynamics of HSV-1 during entry into synaptosomes. Different steps during HSV-1 entry into synaptosomes, for details refer to text. (A–F) Slices of 2.7 to 12.8 nm thickness of various tomographic reconstructions from HSV-1 entry intermediates; (A′ and F′) represent the corresponding schematic drawings. (A and A′) The virus attached to the plasma membrane by interaction of specific glycoproteins with cellular receptors. (B and B′) Fusion pore formation close to the capsid proximal side of the asymmetric virion (see Movie S3 and Fig. 4 for 3D representation). A part of the carbon film is present in the upper left. (C and C′) The capsid entered the cell, and the viral membrane was integrated into the cellular membrane while keeping its curvature, being most prominent in patches studded with glycoprotein spikes. (D and D′) The membrane was partly bent, and most of the tegument remained at the entry site. (E and E′) Glycoproteins were spread along the plasma membrane. (F and F′) The membrane curvature returned to a more flattened, regular organization, and the glycoproteins were removed from the surface. (Scale bars, 100 nm.)