Fig. 2.

ISVP* conversion approximates a second-order reaction at high particle concentrations. (A) ISVPs at the indicated C₀ were subjected to heat treatment at 51, 52, or 53°C for 15 min, then titered by plaque assay. Titer loss is expressed as log₁₀ change relative to a 0°C sample. Points from duplicate experiments are shown with a line connecting mean values. (B) Short time courses bracketing the time required for a 99% reduction in titer (t_99%) were performed over a range of C₀. The expected linear relationship for a second-order reaction is plotted. Means ± SD for three or four determinations are shown with a linear-regression fit. (C) Hemolysis reactions containing ISVPs at the indicated C₀ were performed at 37°C in buffer containing Cs⁺, which facilitates conversion. The expected linear relationship between time to half-maximal hemolysis (t_50%) and concentration is plotted. Means ± SD for four or five determinations are shown with a linear-regression fit line. Examples of time courses used to generate the points in B and C are shown in Fig. S1.