Fig. 4.

μ1N cleavage is required for concentration-dependent ISVP* conversion. WT- or N42A-pRCs at the indicated concentrations were incubated at 37°C in buffer containing Cs⁺, which facilitates conversion. At each time point, a sample was assayed for TRY sensitivity, and the δ remaining after TRY digestion (or μ1δ, in the case of N42A-pRCs) was quantitated. Representative experiments are shown. Gels used for quantitation are shown in Fig. S2.