Fig. 2.

Backbone (¹³C¹H_x)α dipolar spectra for Gly-1, Gly-15, and Gln-16 (Top, Middle, and Bottom). The simulated spectra (Right) were fit against the experimental spectra (Left) in the 2.3- to 10.8-kHz frequency range, and the zero-frequency region was not plotted. The Gly-1 (¹³C¹H₂)α dipolar spectrum order parameter is 0.38 ± 0.03, that for the Gly-15 (¹³C¹H₂)α appears to be ≈ 1.0 but was not successfully simulated, and that for the Gln-16 (¹³C¹H)α dipolar spectrum was 1.01 ± 0.03. Residue Gly-1 is the most dynamic backbone site in our dataset.