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. 2008 Jul 23;105(30):10414–10419. doi: 10.1073/pnas.0803291105

Fig. 3.

Fig. 3.

Cloning of K and LST1 genes. (a) Physical map of the 295-kb region in Chr. 5 of Lotus, where KEW1 was located (upper lane), and the gene structure of LjCYC3 is shown in the lower lane. (b) Phylogenetic tree of TCP family members. CYC (CAA76176) and DICH (AAF12817) are from Antirrhium; PCF1 from rice (BAA23142); LjCYCs from L. japonicus (LjCYC1, ABB36471; LjCYC2, ABB36472; LjCYC3, ABB36473; LjCYC5, ABB36474),and PsCYCs from pea. (c) Genomic Southern blot analysis reveals the deletion of the K gene in k-1 and k-3. (d–f) Expression patterns of PsCYCs in wild type (d) and mutants (e and f). R, root; ST, stems; YL, young leaves; VA, vegetative apex; IM, inflorescence meristem. VIGS phenotype (g–m) side or front view of flowers and lateral or dorsal petals after inoculation are shown. (g) Control, VIGS-GFP. (h and i) VIGS-PsCYC3 in wild-type. (h) VIGS-PsCYC3 flowers with weak k phenotype (arrow) and (i) strong k phenotype (arrow). (j) VIGS-GFP control. (k) VIGS-PsCYC2 in wild-type, dorsal petal with lobes (arrow). (l) VIGS-GFP control in k-1. (m) VIGS-PsCYC1 in k-1, dorsal petal is retarded (arrow).