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. 2008 Jul 23;105(30):10613–10618. doi: 10.1073/pnas.0804536105

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© 2008 by The National Academy of Sciences of the USA

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Fig. 4. — MCH and MCHR1 immunoreactivity in the rat enteric nervous system. (A 1–3) Double staining for MCH and the enteric neuron marker anti-Hu was performed in whole-mount preparations of the rat myenteric plexus. (B 1–3) Double staining for MCH and the enteric neuron marker anti-Hu was performed in whole-mount preparations of the rat submucosal plexus. (C) MCH staining of the guinea-pig myenteric plexus. (D) MCH staining of the guinea-pig submucosal plexus. (E) Negative control staining with the primary antibody for MCH omitted. (F 1–3) Double staining for MCHR1 and anti-Hu in the rat myenteric plexus. (G 1–3) Double staining for MCHR1 and anti-Hu in the rat submucosal plexus. (Calibration bar = 40 μm.)