Fig. 6.

MCHR1 mRNA is expressed in human colonic epithelial cells and increased during inflammation. (A) Colonic epithelial cells from controls (N; n = 7) and patients with CD (n = 5) or UC (n = 4) were isolated from biopsies by LCM, and MCHR1 expression was determined by real-time PCR. (B) HT-29 human colorectal adenocarcinoma cells were treated with IL-1β (10 ng/ml) for 2 h, and MCHR1 expression was evaluated by real-time PCR. (C) HT-29 cells were treated with MCH (10⁻⁶ M) for 3 h, and IL-8 expression was evaluated by real-time PCR.