Fig. 3.
ITS2-DGGE symbiont profiles of seven individual colonies of S. pistillata at three time points: N5 (November 2005, prebleaching), M6 (March 2006, during bleaching), and N6 (November 2006, 8 months postbleaching). March 2005 profiles were identical to November 2005 and are not shown. Symbiont profiles C79 (colonies 1–3) and C35/a (colonies 4 and 5) showed additional bands belonging to symbiont types C8/a, C78, C35/a, or C1unk during bleaching (M6), whereas C8/a and C78 profiles (colonies 6 and 7) remained stable. Representative characteristic bands that were sequenced are marked on the gel, and all sequences belong to known ITS2-DGGE types except C1unk. The lowest C8/a band represents a previously identified pseudogene (C8/a-ps) (29) and HD indicates a heteroduplex formed during PCR. Symbiont designation is shown below each lane; + indicates a symbiont is present other than the original. *C78a was wrongly identified as C1 in ref. 29. Sequence C78a contains a 1bp deletion difference from C1 and occurs in conjunction with C78.