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OriCIvc and oriCIIvc unwinding activity of RctB and DnaA. Various concentrations of RctB (Upper) or DnaA (Lower) were added to reactions that contained 150 fmol of either pBR322 (●), pOriI (■), or pOriII (▲). P1 nuclease was subsequently added. Densitometric scanning of ethidium bromide-stained gels was used to determine the proportion of linearized plasmid molecules. The data presented are representative of at least three experiments.
