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Influence of ATP on RctB binding to oriCIIvc. (A) Schematic representation of the oriCIIvc region, AT-rich region (hatched area), DnaA box (gray rectangle), 11-mer repeats (black triangles), and rctB (arrow). The line below the map represents the probe used for the EMSA experiments. (B) Autoradiographs of EMSA experiments. The indicated molar quantities of RctB were preincubated for 10 min with either ATP or ADP. Afterward, the radioactive probe was added to the reaction.
