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. 2008 Jul 22;105(30):10577–10582. doi: 10.1073/pnas.0803904105

Fig. 6.

Fig. 6.

In vivo characterization of V. cholerae overexpressing R269S RctB. (A) Detection by fluorescent microscopy of oriCIIvc, using TetR-YFP in an N16961 derivative that contains a tet operator array near oriCIIvc. Representative fields after 1 h of induction of TetR-YFP and RctB are shown. (Scale bar: 2 μm.) (B and C) Growth of V. cholerae N1691 with or without overexpressed RctB or R269S RctB. In B, overnight cultures of N16961 harboring a control vector (a and d), a vector containing WT rctB (b and e), or a vector containing R269S rctB (c and f) were serially diluted and spotted onto LB + chloramphenicol (5 μg/ml) with (d–f) or without (a–c) 100 μM IPTG. In C, the overnight cultures were diluted 1,000-fold into fresh media ± IPTG, and growth was monitored by absorbance at 600 nm. Control vector, no IPTG, (open black circles); control vector, + IPTG (filled black circles); WT rctB, no IPTG, (open red triangles); WT rctB + IPTG, (filled red triangles), R269S rctB, no IPTG (open blue diamonds), R269S rctB + IPTG (filled blue diamonds).