Table 2.

In vivo and in vitro vector integration sites distribution

	Intergenic, %	Intragenic, %	Perigenic, %
GLOBE total BM (n = 61)	38.7	53.2	8.1
GLOBE Ter119+ BM (n = 34)	52.9	38.2	8.8
GLOBE Ter119− BM (n = 46)	23.9	65.2	10.9
GLOBE in vitro (n = 57)	27.6	56.9	15.5
LV-GFP in vitro* (n = 202)	28.1	57.9	14.0
Control sequences (n = 61)	49.2	39.3	11.5

Distribution of GLOBE integrations sites unambiguously mapped in total BM, Ter119⁺, and Ter119⁻ sorted cells of th3/+ transplanted mice, and GLOBE and LV GFP integrations in cultured lineage-negative BM cells. Integrations were distributed as inside (intragenic), outside (intergenic), or at a distance of <30 kb upstream or downstream (perigenic) from known genes (University of California Santa Cruz annotation). Control sequences were obtained from a randomly cloned library of MseI/NarI-restricted, LM-PCR-amplified murine BM DNA.

*LV-GFP integrations in lineage-negative BM cells from a previously published collection (10).