Fig. 3.

The three treatments had significantly different effects on nuclear NICD levels in the cortex. (A) Western immunoblot analysis shows nuclear NICD levels from prion-infected mice after treatment with Qa alone, GSI alone, and GSI plus Qa. As controls, nuclear NICD levels in an uninfected mouse (control, C) and an untreated, prion-infected mouse (DMSO) are shown. (B) Quantification of relative nuclear NICD concentrations is given; numbers correspond to treatments described in A. Student's t test probabilities (n = 3 mice each): *, P ≤ 0.05; **, P ≤ 0.01. (C) NICD levels are dissociated from dendritic degeneration. Cortex dendrite load (see Fig. 2D) is graphed as a function of the nuclear NICD concentration shown in B.