Figure 4.

Concentration dependence of the effect of recombinant expressed Pel1 on the extensibility of cucumber hypocotyls. A, Reconstituted expansin-induced extension rates (•) and sustained times (▪) of pretreated basal segments of cucumber hypocotyls varied with Pel1 concentrations in buffer B, pH 5.0. Details are described in Figure 3A. B, The reconstituted expansin-induced extension rates (○) and sustained times (□) of the apical segments varied with Pel1 concentrations in replacing bathing buffer containing 1 mg mL⁻¹ of expansin plus different concentrations of Pel1. Details are described in Figure 1A. Data are means ± se from three independent experiments.