Figure 2.

Mutant alleles of agd1 and ark1. A, Schematic diagrams of the genome organization of ARK1 and AGD1. Boxes indicate exons, and lines indicate introns. The deletion site (agd1-1) and T-DNA insertion sites for each of the mutants are indicated. ark1-3 is a point mutation described by Sakai et al. (2008). B, Semiquantitative RT-PCR of AGD1 and ARK1 in the wild type (WT), agd1-1, agd1-2, agd1-3, and ark1-4. Total RNA was prepared from root tissue, and arrows in A indicate the positions of the primers used for RT-PCR. EIF4A2 primers were used as a control.