Figure 8.

Transient localization of ARK1 and AGD1 in tobacco epidermal cells. A, ARK1-YFP decorates filamentous structures in the cell. B, Incubating cells in the microtubule-depolymerizing drug oryzalin disrupts the filamentous labeling pattern of ARK1-YFP. C, Tobacco leaves inoculated with 35S∷GFP-AGD1 and counterstained with FM4-64. GFP-AGD1 localizes to punctate bodies of varying size that partly overlap with internalized FM4-64 vesicles (arrows). D, Tobacco leaf epidermal cells transiently expressing GFP-AGD1 and mCherry-G-rk. GFP-AGD1 bodies (arrows) do not colocalize with Golgi stacks (arrowheads). Bars = 50 μm (A and B) and 20 μm (C and D).