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. 2008 Aug;147(4):1735–1749. doi: 10.1104/pp.108.122226

Figure 2.

Figure 2.

Yeast two-hybrid interactome of AtPRA1 genes. A to C, Representative mating and β-galactosidase (X-Gal) assay results for AtPRA1.B1 to illustrate the methodology. A matrix was built on microtiter plates containing all prey, which were inoculated by independent bait constructs. The reporter gene encoding GUS was used as a negative control in all experiments. A, Mating assay for AtPRA1.B1. Positive interactions resulting from the activation of the reporter His gene are in gray. B, X-Gal assay for AtPRA1.B1. Positive X-Gal activities are in gray. C, Interaction network summarizing the final result for AtPRA1.B1. Nodes and connecting lines represent AtPRA1 proteins and interactions, respectively. The AtPRA1.B1 node is assigned in black to indicate homodimerization. The interaction was considered as positive only when both His and LacZ reporter genes were activated. D, Interaction network with all AtPRA1 genes. Solid and dashed lines represent interactions in both directions and only one direction, respectively. Noninteractors are listed next to the network.