Figure 4.

Subcellular distribution pattern of AtPRA1 proteins over endosomes in stable transgenic Arabidopsis root cells. Representatives from each clade are presented; for the localization pattern of all AtPRA1 proteins in tobacco, see Supplemental Figure S1. Seedlings were treated for 15 to 30 min with the fluorescent dye FM4-64, which is a reliable tracer for the endocytic trafficking in Arabidopsis root cells (Russinova et al., 2004; Dettmer et al., 2006; Reichardt et al., 2007). A, AtPRA1.A2. B, AtPRA1.B5. C, AtPRA1.D. D, AtPRA1.E. E, AtPRA1.F1. F, AtPRA1.G1. Bars = 10 μm.