Figure 8.
Tissue- and subcellular-based AtPRA1 protein interaction maps. In accordance to the spatial distribution of the 12 AtPRA1 genes observed in 8-d-old Arabidopsis seedlings, the interactome and the subcellular localization data are depicted for every tissue in which the AtPRA1 genes were expressed. Nodes colored black indicate homodimerization. Solid and dashed lines represent interactions in both directions and only one direction, respectively. Interacting proteins localized in the same subcellular compartment are highlighted in orange or green, corresponding to the Golgi and PVC, respectively. Question marks indicate the two AtPRA1 proteins (AtPRA1.F3 and AtPRA1.G2) that showed expression indicative of ER localization, but no strong detectable GFP cells were found to be imaged (data not shown). AtPRA1.D, AtPRA1.F1, and AtPRA1.F3 showed preferential localization over the endosomes/PVC (>PVC). AtPRA1.B1, AtPRA1.B2, AtPRA1.B3, AtPRA1.B4, and AtPRA1.B5 showed preferential localization over the cis-Golgi compartment (>Golgi). AtPRA1.B6 displayed distribution restricted to the ER, while AtPRA1.E showed no preference for the Golgi or endosome compartments (PVC/Golgi).
