Figure 6.

Cortical microtubules of 52-isx are sensitive to 1-butanol. A, Five-day-old GFP-MAP4 untreated. B and C, Five-day-old seedlings carrying GFP:MAP4 were transferred to media containing 0.2% 1-butanol for 22 to 24 h. Some loss of order occurs in MAP4 control (B) but 52-isx (C) is further disorganized by the same treatment, suggesting less stable cortical arrays. (D) Treatment of 52-isx with 0.2% 2-butanol does not affect MT organization demonstrating the specificity of the 1-butanol affects. Scale bar = 10 μm.