Table II.
Down-regulated proteins
The identified proteins are listed in the following table. This table lists the spot number (H, high pI range; L, low pI range), the in-gel and predicted pI and Mr, the averaged ratio between normalized quantities in treated (T) versus control (C) plants, P value (Student's t test) for the replicate groups, and the corresponding accession code (NCBI gi identifier). A protein %CI of ≥95 is considered statistically significant, i.e. there is a 5% or less chance that the match is due to random chance. In cases where liquid chromatography/MS/MS was used to identify the protein, the score and the number of peptides matched (in parentheses) are presented. The significant threshold for Mascot search was set to 0.05. Finally, the function description and the functional category are also presented.
| Spot No. | Mra | pIa | Ratio T/C | P (t Test) | Accession No. | Mrb | pIb | %CI | Function Description | Functional Category |
|---|---|---|---|---|---|---|---|---|---|---|
| L4413 | 26,834 | 5.60 | 0.58 | 0.043 | 17017263 | 84,400 | 5.73 | 99.99 | Met synthase (maize) | Amino acid metabolism, stress response |
| L1107 | 14,231 | 5.35 | 0.28 | 0.000 | 34911874 | 62,777 | 6.22 | 100.00 | Putative ketol-acid reductoisomerase (rice) | Amino acid metabolism |
| H5504 | 36,258 | 6.65 | 0.27 | 0.022 | 22238, 295853 | 36,500 | 6.46 | 100.00 | GADPH, cytosolic: Gpc1 (maize) | Carbohydrate metabolism |
| H3501 | 35,360 | 6.43 | 0.38 | 0.013 | 312179 | 36,519 | 6.41 | 100.00 | GADPH, cytosolic: Gpc2 (maize) | Carbohydrate metabolism |
| H3606 | 40,371 | 6.51 | 0.15 | 0.040 | 6016075 | 36,519 | 6.41 | 100.00 | GADPH, cytosolic: Gpc2 (maize) | Carbohydrate metabolism |
| H5506 | 35,966 | 6.71 | 0.34 | 0.000 | 6166167 | 36,426 | 7.01 | 94.76 | GADPH, cytosolic: Gpc3 (maize) | Carbohydrate metabolism |
| L5510 | 27,312 | 5.75 | 0.49 | 0.001 | 293887 | 24,930 | 8.44 | 99.93 | GADPH, cytosolic: Gpc3 (maize) | Carbohydrate metabolism |
| H4514 | 34,980 | 6.56 | 0.48 | 0.028 | 1184776 | 36,428 | 6.61 | 96.94 | GADPH, cytosolic: Gpc4 (maize) | Carbohydrate metabolism |
| L7105 | 14,967 | 5.96 | 0.57 | 0.024 | 295850 | 38,580 | 7.52 | 97.86 | FBA (maize) | Carbohydrate metabolism |
| L2905 | 103,294 | 5.44 | 0.15 | 0.000 | 8118443Zm.66876 | 24,288 | 5.93 | 77 (3) | Germin-like protein 2, oxalate oxidase (maize) | Carbohydrate metabolism and nutrient reservoir activity and environmental stress response |
| L0908 | 66,070 | 5.30 | 0.37 | 0.000 | 435313 | 64,210 | 6.23 | 900 (32) | β-Glucosidase (maize) | Carbohydrate metabolism, defense against pest signaling (hormone activation) |
| L3308 | 21,803 | 5.49 | 0.38 | 0.001 | 13399869 | 58,371 | 5.52 | 372 (10) | β-Glucosidase, chain B(Zmglu1) (maize) | Carbohydrate metabolism, defense against pest signaling (hormone activation) |
| L3701 | 38,882 | 5.52 | 0.15 | 0.000 | 13399869 | 58,371 | 5.52 | 579 (21) | β-Glucosidase, chain B(Zmglu1) (maize) | Carbohydrate metabolism, defense against pest signaling (hormone activation) |
| L6301 | 22,914 | 5.82 | 0.27 | 0.036 | 435313 | 64,210 | 6.23 | 100.00 | β-Glucosidase (maize) | Carbohydrate metabolism, defense against pest signaling (hormone activation) |
| L6307 | 22,002 | 5.82 | 0.40 | 0.016 | 13399869 | 58,371 | 5.52 | 100.00 | β-Glucosidase, chain B(Zmglu1) (maize) | Carbohydrate metabolism, defense against pest signaling (hormone activation) |
| L2203 | 16,944 | 5.44 | 0.24 | 0.002 | 32490293 | 37,342 | 9.43 | 100.00 | OSJNBa0057M08.27 probable nuclear protein similar to BRUSHY (rice) | Genetic information processing |
| L6806 | 50,267 | 5.85 | 0.42 | 0.001 | 32490293 | 37,342 | 9.43 | 99.97 | OSJNBa0057M08.27 probable nuclear protein similar to BRUSHY (rice) | Genetic information processing |
| L7407 | 26,797 | 6.00 | 0.47 | 0.022 | 32490293 | 37,342 | 9.43 | 97.71 | OSJNBa0057M08.27 probable nuclear protein similar to BRUSHY (rice) | Genetic information processing |
| L7411 | 24,946 | 6.05 | 0.47 | 0.014 | 32490293 | 37,342 | 9.43 | 97.37 | OSJNBa0057M08.27 probable nuclear protein similar to BRUSHY (rice) | Genetic information processing |
| L7514 | 29,992 | 5.95 | 0.57 | 0.026 | 3860254 | 42,284 | 9.88 | 98.59 | Hypothetical protein (At), 95% similar to Golgi GDP Man transporter (GONST1) (Arabidopsis) | Macromolecule metabolic process (protein and lipid glycosylation) |
| L8202 | 17,467 | 6.16 | 0.05 | 0.000 | 56784250 | 8,591 | 10.06 | 99.15 | Unknown protein, DVL-like (rice) | Plant development |
| L7413 | 25,521 | 5.99 | 0.34 | 0.004 | 50948123 | 28,968 | 10.05 | 99.21 | Putative 60s ribosomal protein L7 (rice) | Protein synthesis |
| H6301 | 27,368 | 6.78 | 0.03 | 0.019 | 92868673 | 22,803 | 6.62 | 100.00 | HSP70 (Medicago truncatula) | Protein synthesis, stress response |
| L4105 | 14,909 | 5.63 | 0.41 | 0.039 | 2642648 | 71,448 | 5.09 | 100.00 | Cytosolic heat shock 70 protein HSC70-3 (Spinacia oleracea) | Protein synthesis, stress response |
| L3307 | 22,696 | 5.56 | 0.28 | 0.001 | 1181673 | 22,480 | 7.77 | 100.00 | HSP cognate 70 (Sorghum bicolor) | Protein synthesis, stress response |
| L8505 | 28,059 | 6.32 | 0.46 | 0.000 | 53749463 | 61,201 | 8.19 | 99.95 | Putative TPR domain-containing protein (Solanum demissum) | Protein-protein interaction, function unknown |
| H6601 | 38,334 | 6.82 | 0.10 | 0.045 | 15234171 | 87,056 | 5.06 | 98.42 | Unknown protein (Arabidopsis) | Unknown |
| L1508 | 28,745 | 5.34 | 0.50 | 0.032 | 8809640 | 48,283 | 9.17 | 100.00 | Unnamed protein product (Arabidopsis) | Unknown |
| L3803 | 40,865 | 5.51 | 0.16 | 0.001 | 34897322 | 14,410 | 12.13 | 98.59 | Hypothetical protein (rice) | Unknown |
| L8207 | 16,999 | 6.24 | 0.52 | 0.014 | 20197672 | 49,600 | 8.96 | 99.67 | Unknown protein (Arabidopsis) | Unknown |
| H1615 | 46,120 | 6.35 | 0.57 | 0.025 | No data | |||||
| H2508 | 34,312 | 6.42 | 0.43 | 0.018 | No data | |||||
| H3305 | 27,172 | 6.49 | 0.00 | 0.029 | No data | |||||
| H3401 | 31,731 | 6.44 | 0.36 | 0.016 | No data | |||||
| H3604 | 38,559 | 6.50 | 0.00 | 0.000 | No data | |||||
| H4307 | 25,043 | 6.55 | 0.50 | 0.010 | No data | |||||
| H4604 | 37,200 | 6.54 | 0.19 | 0.023 | No data | |||||
| H5301 | 27,368 | 6.59 | 0.01 | 0.015 | No data | |||||
| H5314 | 27,261 | 6.65 | 0.41 | 0.023 | No data | |||||
| H5317 | 25,694 | 6.65 | 0.13 | 0.006 | No data | |||||
| H6101 | 18,465 | 6.88 | 0.26 | 0.003 | No data | |||||
| H6404 | 31,649 | 6.92 | 0.46 | 0.023 | No data | |||||
| H8301 | 24,610 | 7.22 | 0.44 | 0.021 | No data | |||||
| L2906 | 64,839 | 5.49 | 0.19 | 0.001 | No data | |||||
| L3303 | 23,062 | 5.55 | 0.51 | 0.000 | No data | |||||
| L5809 | 44,055 | 5.71 | 0.30 | 0.008 | No data | |||||
| L4511 | 27,492 | 5.65 | 0.43 | 0.003 | No data | |||||
| L7308 | 21,675 | 6.02 | 0.19 | 0.003 | No data | |||||
| L8407 | 25,529 | 6.28 | 0.44 | 0.000 | No data | |||||
| L8704 | 37,918 | 6.19 | 0.27 | 0.000 | No data |
a
In-gel pI and Mr.
b
Predicted pI and Mr.