Figure 4.

A and B, 2-DE analysis of soluble proteins (340 μg) isolated from laminae of young leaves of plants treated with MeJA at 7 d after TI detection (A) or silver staining (B). Arrows indicate TI activity and two spots (spot nos. 1 and 2) corresponding with the TI activity are numbered. Identified spots were excised and digested with trypsin and analyzed by ESI-LC MS/MS. C, Alignment of 218-amino acid sequence of BnD22 (accession no. X65637) and sequenced peptides A and B by ESI-LC MS/MS from each spot. The shaded amino acid is shared by peptides A and B. Amino acid sequence of the motif of Künitz-type protease inhibitor is surrounded (Nishio and Satoh, 1997).