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Silver-stained 2-DE gels of total proteins from laminae of young leaves of oilseed rape supplied (A) or not (B) with nitrate at 21 d; 125 μg of total protein was loaded on an IEF strip (for details see “Materials and Methods”). Two spots corresponding with BnD22 are numbered as 1 and 2. Molecular mass markers (kD) are listed on the left of the gel.
