Figure 6.

A and B, Silver-stained 2-DE of total proteins from laminae of young leaves of control plants (A) and plants treated with MeJA (B) during 7 d. A total of 125 μg of total protein was loaded on an IEF strip (for details see “Materials and Methods”). Molecular mass markers (kD) are listed on the left of the gel. C, 2-DE immunoblot from the lamina of a plant treated with MeJA using anti-WSCP antibodies. Total proteins were transferred to a PVDF membrane before immunodetection using anti-WSCP antibodies. Only the region of the 2-DE gel displaying the protein after silver staining is shown in D. This region is surrounded by a box in B. Polypeptides induced by MeJA and/or recognized by the antibodies are numbered from 1 to 9, excised, digested, and identified by ESI-LC MS/MS (see Tables II and III).