Figure 1.
Analysis of radioactive compounds in methanolic extracts of dissected cambium and associated developing secondary xylem from lodgepole pine following 4 h of incubation in [3H]Phe. A, HPLC results show separation of phenolic compounds, detected by their UV absorption at 280 nm and identified by coelution with standards and LC-MS. B, Radioactivity from each HPLC fraction above was collected every 30 s, and the amount of radiolabel in the fraction was measured by scintillation counting. Peaks correspond to the monolignols p-coumaryl alcohol and coniferyl alcohol at 8 min of elution, pinoresinol at 14.5 min, and a large peak at 11 min corresponding to three peaks at 10 to 11 min that gave complex MS fragmentation patterns, including coniferyl alcohol and p-coumaryl alcohol fragments.