Figure 5.

Quantification of radioactive label in developing tracheids of pine following cryofixation, autoradiography, and TEM. A TEM autoradiography quantification example is shown (control, [³H]Phe only). The density of the decay events was measured by drawing probability circles with radii of 500 nm, which would capture 90% of possible sources around a decay event. The area of the underlying organelle that intersected the probability circle was calculated. CW, Cell wall; G, Golgi; V, vacuole. Bar = 2 μm.