Figure 10.

The T-DNA region of the pART27-lfsRNAi-mgfp5ER silencing cassette used to silence LFS in onion. A 521-bp DNA fragment derived from lfs cDNA (AB089203) at positions 56 to 576 was selected for a sense lfs region of an RNAi construct and inserted into the XhoI and KpnI sites in pHANNIBAL. Similarly, a 512-bp DNA fragment at positions 63 to 574 was selected for an antisense lfs region and inserted into the ClaI and BamHI sites. The RNAi construct was excised from pHANNIBAL using the restriction enzyme NotI and inserted into the NotI site in the pART27-mgfp5ER. The gfp marker gene was used to aid the selection of transgenic events. LB, Left border; RB, right border.