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. 2008 Aug;147(4):2096–2106. doi: 10.1104/pp.108.123273

Figure 3.

Figure 3.

Molecular analysis. Top, Southern analysis of lfsRNAi transgenic onion plants probed with gfp probe. Center, RNAi analysis of small RNA probed with lfs probe. Bottom, Quantitative RT-PCR measurement of lfs transcript compared with nontransgenic (NT). HN, ON, and DN indicate nontransgenic hybrid, open-pollinated, and dehydration control, respectively (stars); H1, H2, H3, O1, O2, O3, D1, and D2 represent specific transgenic hybrid, open-pollinated, and dehydration onion plants (circles). Far right lanes show one-, five-, and 10-copy control, respectively (on Southern-blot gel) and LFS sequence control (on RNAi gel). The 95% confidence limit is calculated from an ANOVA of logged data using the within-bulb variation. Data were log-transformed to adjust for variance heterogeneity between lines.