Table I.
Analysis | Plants
|
|||||||
---|---|---|---|---|---|---|---|---|
H1 | H2 | H3 | O1 | O2 | O3 | D1 | D2 | |
PCR | Intact T-DNA | CaMV 35S deleted | Intact T-DNA | Intact T-DNA | Intact T-DNA | nptII deleted | Intact T-DNA | Intact T-DNA |
Southern blot | Two at different loci | Single insert | Single insert | Multiple inserts at one locus | Single insert | Single insert | Two at different loci | Single insert |
lfsRNAitranscripts | Present | Present | Present | Absent | Present | Present | Present | Present |
siRNAs | Present | Present | Present | Absent | Present | Present | Absent | Present |
lfs transcripts | Reduced | Reduced | Reduced | Normal | Reduced | Reduced | Normal | Reduced |
LFS protein | Reduced | Reduced | Reduced | Normal | Reduced | Reduced | Normal | Reduced |
LFS activity | Reduced | Reduced | Reduced | Normal | Reduced | Reduced | Normal | Reduced |