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. 2008 Aug;147(4):2096–2106. doi: 10.1104/pp.108.123273

Table I.

Summary of molecular and biochemical data for transgenic onion plants

Analysis Plants
H1 H2 H3 O1 O2 O3 D1 D2
PCR Intact T-DNA CaMV 35S deleted Intact T-DNA Intact T-DNA Intact T-DNA nptII deleted Intact T-DNA Intact T-DNA
Southern blot Two at different loci Single insert Single insert Multiple inserts at one locus Single insert Single insert Two at different loci Single insert
lfsRNAitranscripts Present Present Present Absent Present Present Present Present
siRNAs Present Present Present Absent Present Present Absent Present
lfs transcripts Reduced Reduced Reduced Normal Reduced Reduced Normal Reduced
LFS protein Reduced Reduced Reduced Normal Reduced Reduced Normal Reduced
LFS activity Reduced Reduced Reduced Normal Reduced Reduced Normal Reduced