Figure 9.

PR-1 and PDF1.2 gene expression and SA/SAG levels. A, Northern-blot analysis of PR-1 gene expression in Col-0, gly1, and act1 plants spray inoculated with water or 10⁶ spores/mL of C. higginsianum. The samples were collected at 3 dpi. RNA gel-blot analysis was performed on approximately 7 μg of total RNA extracted and ethidium bromide staining of rRNA was used as a loading control. B, SA levels in Col-0, gly1, and act1 plants inoculated with water or 10⁶ spores of C. higginsianum. The plants were spray inoculated and the samples were collected at 3 dpi. C, SAG levels in Col-0, gly1, and act1 plants inoculated with 10⁶ spores of C. higginsianum. The plants were spray inoculated and the samples were collected at 3 dpi. D, Northern-blot analysis of PDF1.2 gene expression in Col-0 plants spray inoculated with water (mock) or 10⁶ spores/mL of C. higginsianum. The samples were collected at 3 dpi. RNA gel-blot analysis was performed on approximately 7 μg of total RNA extracted and ethidium bromide staining of rRNA was used as a loading control.